Composite

Part:BBa_M36584:Design

Designed by: Aris Kare, Eda Benites, Ricky Cordova   Group: Stanford BIOE44 - S11   (2015-10-24)

Making acetoacetate from acetic acid and acetoacetyl-CoA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 91
    Illegal XbaI site found at 772
    Illegal PstI site found at 841
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 91
    Illegal PstI site found at 841
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 91
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 91
    Illegal XbaI site found at 772
    Illegal PstI site found at 841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 91
    Illegal XbaI site found at 772
    Illegal PstI site found at 841
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Chose to use only one promoter for both ctfA and ctfB to shorten the length of the plasmid. Strong RBS stitches ctfA and ctfB together.


Source

Sequence for ctfA obtained from http://www.ncbi.nlm.nih.gov/gene/1116168 Sequence for ctfB obtained from http://www.ncbi.nlm.nih.gov/gene/1116169

References